Coronavirus Information for the UC San Diego Community

Our leaders are working closely with federal and state officials to ensure your ongoing safety at the university. Stay up to date with the latest developments. Learn more.

A comparison of isogenic homozygous clone and wildtype zebrafish (Danio rerio): Survival and developmental responses to low pH conditions

TitleA comparison of isogenic homozygous clone and wildtype zebrafish (Danio rerio): Survival and developmental responses to low pH conditions
Publication TypeJournal Article
Year of Publication2020
AuthorsAdams L.G, Gordon M.S, Buth D.G, Hutchings E.M
Date Published2020/04
Type of ArticleArticle; Early Access
ISBN Number1545-8547
Accession NumberWOS:000528403500001
Keywordsacid; bioassay; clone; Developmental Biology; ecotoxicology; environmental stress; ethanol exposure; fish; genetic-variation; isogenic; populations; rainbow-trout; stability; strains; tolerance; water; zoology

The value of bioassays as analytical methods for assessing the potency of particular stressors on live animal models depends on the precision of their results, which are greatly influenced by the choice of test subjects. The genetic makeup of experimental subjects varies, and, as such, so will their responses to the test environment. Genetic diversity of test populations may contribute to statistical variability; therefore, the use of genetically similar subjects may enhance the utility of bioassays. This study addresses the efficacy of using isogenic homozygous zebrafish (Danio rerio) as subjects for bioassays. Stress responses (acidic conditions) were compared during early development for gynogenetically produced isogenic homozygous line of zebrafish (C32) and wildtype (WT) zebrafish. Experiments evaluated early life stage milestones after exposure to low pH in water of a different electrolyte composition. Because the isogenic homozygous clonal (IHC) fish possessed far less genetic variability than the WT fish tested, it was predicted that the IHC fish would exhibit less variability in their response to stress. Although we found no significant differences in the variability between the responses of the IHC and WT fish, pH and water hardness level had a differential effect on the two groups. Simple strain differences may be the probable cause of the response differences to environmental stress. Factors that may affect stress response, such as heterogeneity, co-adapted gene complexes, and domestication, are discussed. Our findings and review of recent zebrafish literature stress the need for researchers to carefully consider breeding histories and trait characteristics for each potential test subject to maximize the sensitivity of the assay.

Student Publication: