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Genetic tool development in marine protists: emerging model organisms for experimental cell biology

TitleGenetic tool development in marine protists: emerging model organisms for experimental cell biology
Publication TypeJournal Article
Year of Publication2020
AuthorsFaktorova D., Nisbet R.ER, Robledo J.AF, Casacuberta E., Sudek L., Allen A.E, Ares M., Areste C., Balestreri C., Barbrook A.C et al.
Date Published2020/04
Type of ArticleArticle; Early Access
ISBN Number1548-7091
Accession NumberWOS:000523954200003
Keywordsalga; Biochemistry & Molecular Biology; genome; homologous recombination; transformation

Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways. This Resource describes genetic tools for microbial eukaryotes, providing a roadmap for developing genetically tractable organisms.

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